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Objective To investigate the changes of GP130 expression in rats with pressure overload-induced cardiac hypertrophy, and the effect of Benazepril on GP130 expression and the concerned cardiac hypertrophy. Methods Two weeks after the Wistar rat model of pressure overload was established by constriction of abdominal aorta, the survived rats were randomly divided into hypertrophy group (LVH, n=7) and Benazepril intervention group (Ben, n=7, 1 mg?kg -1 ?d -1 Benazepril orally for 3 weeks). A sham-operation group (Sham, n=7) was set up as control. Blood pressure and left ventricular mass index (LVMI) were investigated at 3th week after model establishment. AngⅡ level in myocardium was measured by radioimmunoassay. The protein level of GP130 in cardiomyocytes was determined by immunohistochemistry. Results As compared with the Sham group, blood pressure and LVMI increased significantly (P
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Objective To investigate the changes of cardiotrophin-1(CT-1) expression in rats with pressure overload-induced cardiac hypertrophy,and the effects of benazepril on this expression and the concerned cardiac hypertrophy.Methods The model of pressure overload was established by constriction of abdominal aorta and divided randomly into hypertrophied group(LVH,n=7) and benazepril intervention group(Ben,n=7,benazepril 1mg?kg~(-1)?d~(-1) orally)2 weeks later.A sham-operation group(Sham,n=7) served as control.Blood pressure and left ventricular mass index(LVMI) were investigated after 3 weeks' treatment.AngⅡ level in myocardium was measured by radioimmunoassay.The mRNA level of CT-1 was examined by RT-PCR.Results Compared with the sham group, blood pressure and LVMI in LVH group were increased significantly(P
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Objective To study the reversion of the metabolic gene expression pattern of hypertrophic cardiac and role of Carvedilol and to explore the molecular regulatory mechanism of Carvedilol on attenuating the reversion back towards fetal energy metabolism occurring with the development of cardiac hypertrophy. Methods A model of hypertrophy induced by coarctation of abdominal aorta(CAA) in male Wistar rats was employed and changes of parameters such as hemodynamics, ventricular remodeling parameters, free fatty acid in blood serum and cardiac myocyte and expressions of muscle carnitine palmitoyltransferase Ⅰ (M CPT Ⅰ) and medium chain acyl CoA dehydrogenase (MCAD) mRNA were investigated in the experimental animals in operation group(CAA), sham operation group(SH) and Carvedilol intervention group(CAR) at 16 weeks after operation. Results Significant hypertrophy was found in the left ventricle in CAA group(3.41?1.30 vs 2.46?1.31, P
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Objective To study the mechanism of carvedilol therapy of congestive heart failure. Methods After chronic heart failure model was established by ligation of the left coronary artery in rats, rats were treated with carvedilol and terazosin. Then hemodynamic parameters, activity of sarcoplasmic reticulum(SR) Ca 2+ pump(SERCA 2a ) and the rate of cardiomyocyte apoptosis were determined. Results Compared with those of the control group( group C), the activity of SR Ca 2+ pump in the heart failure group(group F) decreased( P 0.05). Carvedilol intervention reduced the rate of cardiomyocyte apoptosis, but enhanced the activity of SR Ca 2+ pump significantly in dose dependent manner. The activity of SR Ca 2+ pump was negatively correlated with the rate of cardiomyocyte apoptosis( r =-0.814, P
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Objective To study the effects of ACE1 on amount and mRNA expression of Ca2+ release channels(RyR2) of myocardial sarcoplasmic reticulum(SR) in prevention and treatment of chronic heart failure.Methods After the model of chronic heart failure was established with ligation of left corongary artery in rats,the animals were prevented and treated with Perindopril. Hemodynamic parameters, Bmax and Kd of ［3H］－ryanodine binding to RyR2、RyR2 mRNA content were determined.Results Compared with the control group(group C),LVEDP in the heart faliure group (group F)increased(P0.05).Conclusion The amount and mRNA expression of RyR2 of myocardial sarcoplasmic reticulum(SR) decreased in chronic heart failure.Perindopril can improve mRNA expression and amount of RyR2 of myocardial SR in prevention and treatment of chronic heart failure, thus contributing to the improvement of myocardial function.
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Objective The aim of this study was to asses the success rate, lesion characteristics and complication in acute coronary syndromes treated by direct coronary stenting Methods 92 patients with acute myocardial infarction and unstable angina were divided into the direct stenting ( n =32) and stent implantation with balloon predilatation (conventional stenting, n =60) groups The clinical data, characteristics of target vessels ,success rate and complications were compared between two groups Results The age in direct stenting groups was much younger than that in conventional stenting group (53 14?9 18 vs, 64 28?12 36, P
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The mammalian fetal heart relies primarily on glucose and pyruvate as substrates for ATP production, and it is rapidly transformed to fatty acid ?-oxidation (FAO) postnatally. However, energy metabolic pathways revert to a fetal pattern, when cardiac hypertrophy and heart failure developed. This return was called recapitulation, ultimately it is maladaptive for the body. The process of FAO was performed under a precise regulating system, nuclear transcription factors such as PPAR?, Sp1/3, Coup-TF(chicken ovalbumin upstream promoter transcription factor) all take part in the regulation of genes transcription of FAO enzymes. It was identified that the reguction of PPAR? activity in hypertrophic myocardium due to pressure overload might result in down-regulation of gene expression of FAO enzyme. The mechanism involved in reinduction of a fetal gene transcription participated in the regulation of myocardial energy metabolism in the development of cardiac hypertrophy resulting from pressure overload.
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AIM:To study the effects and mechanism of peroxisome proliferator-activated receptors(PPARs)ligands,fenofibrate and pioglitazone,on ventricular remodeling in pressure overload rats.METHODS:A pressure overload model was established by the constriction of abdominal aorta in Wistar rats.The hemodynamics and ventricular remodeling parameters,plasma and myocardial renin activity,angiotensin Ⅱ and aldosteron,the mRNA expression of angiotensin Ⅱ type 1 receptor(AT1)were investigated in the constriction of abdominal aorta group(CAA group,n=7)at 12-week after operation and treated experimental groups in which rats were treated with fenofibrate(F group,n=8),pioglitazone(P group,n=7),concomitant fenofibrate and pioglitazone(F+P group,n=6)for 12 weeks since 2 days after operation.The sham-operated rats served as controls(n=8).RESULTS:The ratio of left ventricular weight to body weight,mean arterial pressure,left ventricular systolic pressure,left ventricular end diastolic pressure,left ventricular systolic pressure and heart rate were significantly lower,the maximum left ventricular pressure rising and declining rates(?dp/dtmax)were significantly higher in all treated experimental groups than those in CAA group.Fenofibrate or pioglitazone had no effect on plasma and myocardial levels of renin,angiotensin Ⅱand aldosteron.The mRNA expression of AT1 was downregulated in treated groups except F group.CONCLUSION:PPAR ligands have no effect on plasma and myocardial levels of renin,angiotensin Ⅱand aldosteron,but fenofibrate and pioglitazone inhibit ventricular remodeling,decrease preload and afterload,increase ?dp/dtmax in pressure overload rats.The expression of mRNA of AT1 is downregulated in myocardium of pressure overload rats by the PPAR? signaling pathway.
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AIM: To research clinical effect of curing patients with CHF(congestive heart failure) with Metoprolol and Shexiang Baoxing Pills(SBP). METHODS: 156 CHF patients were divided into three groups,including M(Metoprolol),SBP and M-SBP at random.The first dosage of Metoprolol was specified by the heart function,taking SBP 3 times per day and 2 pieces once,totally 8 weeks.Observeing Plasma Cyclic Nucleotide(Cyclic Adenosine monophosphate and Cyclic Guanosine Monophosphate),Norepinephrine(NE),Atrial Natriuretic Peptide(ANP) and Heart Ejection Fraction(EF),Cardiac Output(CO),heart and chest proportion,Before and after the curing. RESULTS: The curing effect of M-HMP group obviously surpasses simply M group and HMP group. CONCLUSION: Curing CFH patients by metoprolol assistant with SBP is better method.
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Objective To explore the expression of peroxisome proliferator activated receptor? (PPAR?) and its roles in the differentiation of mesenchymal stem cells (MSCs) induced by 5 Azacytidine. Methods 5 Azacytidine was used to induce the differentiation of primary cultured mesenchymal stem cells. The eukaryotic expression plasmid vector pEGFP N1 PPAR? was transfected into MSCs with lipotransfection method followed by G418 selection. The expression levels of PPAR? mRNA were detected by RT PCR. Immunohistochemistry and Western blot were employed to study the protein expression of PPAR?. Lipid droplets in the cells were stained with Oil Red O. Results No expression of PPAR? mRNA was found in undifferentiated MSCs. After induction by 5 Azacytidine, partial MSCs expressed PPAR? and differentiated into lipoblasts. MSCs, transfected with pEGFP N1 PPAR?2, differentiated into adipocytes. Conclusion 5 Azacytidine can induce the differentiation of MSCs into myoblasts and lipoblasts, which may be related to the expression of PPAR?.
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AIM: To study changes of medium chain acyl- Co A dehydrogenase (MCAD), muscle carnitine palmitoyltransferase I (M-CPT-I) and co lligin mRNA/protein expression, to elucidate molecular mechanism of the recapit ulation of fetal energy metabolism and ventricular remodeling and the effects o f carvedilol during the development of pressure overload-induced left ventricula r hypertrophy in rats. METHODS: Male Wistar rats of hypertrophy induced by constrictio n of abdominal aorta (CAA) were randomized into 2 groups (n=12, each group): 4-week group (CAA 4 weeks group) and 12-week carvedilol intervention gro up (CAR group). Additi onal rats (n=12) underwent abdominal cavity incision without ligation to ser ve as age-matched sham operated controls (SH). Hemodynamics, ventricular remodel ing parameters and free fatty acid (FFA) both in blood serum and myocardium we re measured. RT-PCR analysis of the expression of mRNA of M-CPT-I, MCAD and col lagen binding protein (colligin) were investigated. The protein expression of co lligin was analyzed by Western blotting in the experimental animals and sham op eration.RESULTS: LVM/BW and MAP in CAA group were increased more signif icantly than in sham group. There were progressive increases in FAA both in bloo d serum and myocardium in CAA group than in sham group, accompanied with downreg ulation of gene expressions of M-CPT-I and MCAD and colligin mRNA/ protein upre gulation in LV in CAA group, while changes of all of these parameters in CAR gro up were attenuated. CONCLUSIONS: (1) The down-regulated expression of cardiac FAO en zyme genes (M-CPT-I and MCAD) in the hypertrophied heart may be responsible for "the recapitulation of fetal energy metabolism" during the development of pres sure overload-induced left ventricular hypertrophy in rats. (2) Carvedilol atten uates the reversion of the metabolic gene expression back towards fetal type. (3 ) Carvedilol is effective in regressing the left ventricular remodeling by inhi biting colligin protein expression. A molecular mechanism by which carvedilol ma y confer cardioprotective effects in heart failure may be, in part, via preservi ng o f the adult metabolic gene regulation and regressing left ventricular remodeling .
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Forty-eight cases of atrial septum secundum defect were repaired and followed up with M-mode echocardiography postoperatively for three months to three yaers respectively.All the cases showed a significant reduction of the inner diameter of the right ventricle after operation,and in 23% of the cases, the inner diameter even returned to the normal range. In 92% of the acses, the intervetricular septal paradoxical motion resumed the normal pattern. In most cases, the inner diameter of the left ventricle increased markedly.In 11 cases with repeated follow-up studies, it was found that three months after operation, the interventricular septal paradoxical motion became normal in 7 cases and the motion flattened in three cases. In 18 cases with a 3-year follow-up history, it was found that the recovery of the heart condition showed no relation to the age of the patient. This is likely to suggest that the age factor exerts no influence on the postoperative recovery of the cases having the atrial septal defect repaired.
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Fifty cases with chronic rheumatic valvular disease were examined with two dimensional echocardiography to evaluate the valvular pathology preoperatively. The echocardiographic findings were correlated and compared with the operative findings.It was found that 89% of the thickening pattern cases and 75% of the funnel-shaped cases showed thickening of the leaf-lets- All the 28 membranous cases showed diastolic doming and restricted tip motion of the anterior leaflet in different degrees. In the 4 funnel-shaped cases, 3 showed restricted tip and body motion of the leaflets and the 4th case showed stiffness of the leaflet base. 63% of the mitral regurgitation cases were confirmed at operation.It is concluded that preoperative evaluation of the valvular pathology with two dimensional echocardiography is helpful in selection of suitable operative candidates.